Time course changes of oxidative stress markers in a rat experimental glaucoma model.
نویسندگان
چکیده
PURPOSE To evaluate the relationship between oxidative stress markers and increased intraocular pressure in experimental glaucoma. METHODS In vivo chemiluminescence (CL), total antioxidant capacity (TRAP), nitrite concentration (NC), and lipid peroxidation markers (TBARS) were evaluated. Wistar rats (n=18 for each time point) underwent operation, and two episcleral veins were cauterized. RESULTS Decreases of 22%, 35%, and 27% at 7, 15, and 30 days and an increase of 22% at 60 days in CL were observed in glaucomatous eyes. In optic nerve, TBARS values were 6.9+/-0.5 nmol/mg protein (7 days), 9.4+/-0.4 nmol/mg protein (15 days), 18.0+/-1.2 nmol/mg protein (30 days), and 43.1+/-5.3 nmol/mg protein (60 days) (control, 6.2+/-0.4 nmol/mg protein; P<0.001). NC was 37.0+/-1.8 microM (7 days), 31.4+/-1.2 microM (15 days), 39.6+/-1.3 microM (30 days), and 40.0+/-1.3 microM (60 days) (control, 21.1+/-1.7 microM; P<0.001). In glaucomatous vitreous humor, TRAP decreased by 42% at 15 days and 78% at 60 days (control, 414+/-29 microM; P<0.001). In glaucomatous aqueous humor, TRAP values were 75+/-7 microM (7 days), 54+/-4 microM (15 days), 25+/-4 microM (30 days), and 50+/-3 microM (60 days) (control, 90+/-10 microM; P<0.001). CONCLUSIONS Reactive species were increased in glaucoma, as evidenced by the increases in CL, TBARS, and NC. The decrease in the antioxidant levels may be a consequence of an increase in oxidative processes.
منابع مشابه
Antidiabetic effect of honey feeding in noise induced hyperglycemic rat: involvement of oxidative stress
Objective(s):In this study the effect of oral administration of honey on serum glucose, lipids, stress oxidative markers, and morphology of langerhans islets in noise induced hyperglycemic rats was investigated. Materials and Methods: Male Wistar rats were divided into control, hyperglycemic, honey treated control, and honey treated hyperglycemic groups. For induction of hyperglycemia, noise s...
متن کاملRetracted: Time course changes of oxidative stress and inflammation in hyperoxia-induced acute lung injury in rats
متن کامل
Changes of stress oxidative enzymes in rat mammary tissue, blood and milk after experimental mastitis induced by E. coli lipopolysaccharide
The present study investigated the effects of E. coli lipopolysaccharide (LPS) induced mastitis model in rat on the activity of antioxidant enzyme systems. To achieve this purpose, E. coli LPS were infused into the mammary gland of 12 rats 72 hr postpartum and compared with 12 rats in control group infused intramammary placebo sterile pyrogene – free, physiological saline. The...
متن کاملThe Effect of a Course of Aerobic Exercise With Hypoallergenic Supplement of Tribulus Terrestris on Mitochondrial Oxidative Stress Markers of Cardiac Tissue in Rats Toxicated With Hydrogen Peroxide
Introduction: Oxidative stress causes DNA damage and apoptosis and leads to cardiovascular disease. The aim of this study was to evaluate the effect of a course of aerobic exercise with hypoallergenic supplement of Tribulus terrestris on mitochondrial oxidative stress markers of cardiac tissue in rats toxicated with hydrogen peroxide. Method: To implementation of this experimental research, 4...
متن کاملTime course changes of oxidative stress and inflammation in hyperoxia-induced acute lung injury in rats
Objective(s):Therapies with high levels of oxygen are commonly used in the management of critical care. However, prolonged exposure to hyperoxia can cause acute lung injury. Although oxidative stress and inflammation are purported to play an important role in the pathogenesis of acute lung injury, the exact mechanisms are still less known in the hyperoxic acute lung injury (HALI). Materials ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Investigative ophthalmology & visual science
دوره 51 9 شماره
صفحات -
تاریخ انتشار 2010